Journal: Journal of Cell Science

Article Title: Inhibition of Notch signaling by the p105 and p180 subunits of Drosophila chromatin assembly factor 1 is required for follicle cell proliferation

doi: 10.1242/jcs.224170

Figure Lengend Snippet: Inactivation of CAF1-p105/p180 results in induction of Notch target gene expression in follicle cells at the mitotic stage. (A–A‴) Induction of Hnt expression was detected in homozygous CAF1-p10536 mutant follicle cell clones (GFP-negative in A′) at the mitotic stage. (B–B‴) Induction of Hnt expression was detected in homozygous CAF1-p10536 MARCM mutant follicle cell clones (GFP-positive in B′) at the mitotic stage. (C–C‴) Induction of Hnt expression was detected in CAF1-p180 RNAi-expressing follicle cell clones (GFP-positive in C′) at the mitotic stage. (D–D‴) Induction of E(spl)m7-LacZ expression was detected in homozygous CAF1-p10536 mutant follicle cell clones (GFP-negative in D′) at the mitotic stage. (E–E‴) Induction of E(spl)mβ-CD2 expression was detected in CAF1-p180 RNAi#1-expressing follicle cell clones (GFP-positive in E′) at the mitotic stage. (F–F‴) Induction of E(spl)mβ-CD2 expression was detected in CAF1-p180 RNAi#2-expressing follicle cell clones (GFP-positive in F′) at the mitotic stage. Hnt staining is shown in A,B,C. β-galactosidase (LacZ) staining is shown in D and CD2 staining is shown in E,F. DNA staining with DAPI is shown in A″,B″,C″,D‴,E‴,F‴. The genotypes of GFP-negative and GFP-positive cell clones are indicated in black and green boxes, respectively, on the left sides of image panels. Scale bars: 10 μm.

Article Snippet: The following antibodies were used: mouse anti-Cut, 1:15 (2B10); mouse anti-Eya, 1:10 (eya10H6); mouse anti-Hnt (1G9), 1:15 (1G9); mouse anti-N ICD , 1:50 (C17.9C6) (all from the Developmental Studies Hybridoma Bank; DSHB); rabbit anti-PH3, 1:200 (catalog no. 06-570, Millipore); rabbit anti-β-galactosidase, 1:5000 (catalog no. 08559761, MP Biomedicals) and mouse anti-CD2, 1:50 (catalog no. MCA154G, AbD Serotec).

Techniques: Expressing, Mutagenesis, Clone Assay, Staining

Journal: Journal of Cell Science

Article Title: Inhibition of Notch signaling by the p105 and p180 subunits of Drosophila chromatin assembly factor 1 is required for follicle cell proliferation

doi: 10.1242/jcs.224170

Figure Lengend Snippet: Inactivation of CAF1-p105/p180 has no impact on Notch target gene expression in follicle cells at the endocycle stage. (A–A‴) Homozygous CAF1-p10536 mutant follicle cell clones (GFP-negative in A) at the endocycle stage manifested Hnt expression similar to that observed in wild-type and heterozygous cell clones. Hnt staining is shown in A′. (B–B‴) CAF1-p180 RNAi-expressing follicle cell clones (GFP-positive in B) at the endocycle stage manifested normal Hnt expression as wild-type cell clones. Hnt staining is shown in B′. (C–C‴) Homozygous CAF1-p10536 mutant follicle cell clones (GFP-negative in C) at the endocycle stage manifested E(spl)m7-LacZ expression similar to that observed in wild-type and heterozygous cell clones. β-galactosidase (LacZ) staining is shown in C′. DNA staining with DAPI is shown in A″,B″,C″. The genotypes of GFP-negative and GFP-positive cell clones are indicated in black and green boxes, respectively, on the left sides of image panels. Scale bars: 10 μm.

Article Snippet: The following antibodies were used: mouse anti-Cut, 1:15 (2B10); mouse anti-Eya, 1:10 (eya10H6); mouse anti-Hnt (1G9), 1:15 (1G9); mouse anti-N ICD , 1:50 (C17.9C6) (all from the Developmental Studies Hybridoma Bank; DSHB); rabbit anti-PH3, 1:200 (catalog no. 06-570, Millipore); rabbit anti-β-galactosidase, 1:5000 (catalog no. 08559761, MP Biomedicals) and mouse anti-CD2, 1:50 (catalog no. MCA154G, AbD Serotec).

Techniques: Expressing, Mutagenesis, Clone Assay, Staining

Journal: Journal of Cell Science

Article Title: Inhibition of Notch signaling by the p105 and p180 subunits of Drosophila chromatin assembly factor 1 is required for follicle cell proliferation

doi: 10.1242/jcs.224170

Figure Lengend Snippet: Cut is required for silencing Hnt expression in mitotic follicle cells. (A–A‴) Immunostaining of Cut was performed to examine the knockdown efficiency of the Cut RNAi in mitotic follicle cells. GFP-positive Cut RNAi-expressing cell clones are shown in A′. Cut staining is shown in A. (B–B‴) Hnt expression was induced in Cut RNAi-expressing follicle cell clones (GFP-positive in B′) at the mitotic stage. Hnt staining is shown in B. (C–C‴) Expression of E(spl)m7-LacZ was not induced in Cut RNAi-expressing follicle cell clones (GFP-positive in C′) at the mitotic stage. β-galactosidase (LacZ) staining is shown in C. (D–D‴) Immunostaining of Cut was performed to examine the expression of UAS-Cut in CAF1-p180 RNAi-expressing follicle cell clones (GFP-positive in D′). Cut staining is shown in D. (E–E‴) Exogenous expression of Cut abolished the induction of Hnt expression in CAF1-p180 RNAi-expressing follicle cells (GFP-positive in E′) at the mitotic stage. Hnt staining is shown in E. DNA staining with DAPI is shown in A″,B″,C″,D″,E″. The genotypes of GFP-negative and GFP-positive cell clones are indicated in black and green boxes, respectively, on the left sides of image panels. Scale bars: 10 μm.

Article Snippet: The following antibodies were used: mouse anti-Cut, 1:15 (2B10); mouse anti-Eya, 1:10 (eya10H6); mouse anti-Hnt (1G9), 1:15 (1G9); mouse anti-N ICD , 1:50 (C17.9C6) (all from the Developmental Studies Hybridoma Bank; DSHB); rabbit anti-PH3, 1:200 (catalog no. 06-570, Millipore); rabbit anti-β-galactosidase, 1:5000 (catalog no. 08559761, MP Biomedicals) and mouse anti-CD2, 1:50 (catalog no. MCA154G, AbD Serotec).

Techniques: Expressing, Immunostaining, Clone Assay, Staining